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vsv g envelope protein  (Addgene inc)


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    Structured Review

    Addgene inc vsv g envelope protein
    Vsv G Envelope Protein, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 3069 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vsv+g+envelope+proteins+plasmid/pCMV-VSV-G+(Plasmid+%238454)/pm41922576-124-21-25
    Average 96 stars, based on 3069 article reviews
    vsv g envelope protein - by Bioz Stars, 2026-09
    96/100 stars

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    Incubation:

    Article Title: MER57E3 transposable elements regulate gene expression in a human cell model of neural development.
    Article Snippet: .. Upon 2 hour incubation with Chloroquine-supplemented media (High-glucose DMEM (Gibco) supplemented with non-essential amino acids (NEAA, Gibco, 1x), 10% FBS (Gibco), GlutaMAX (2 mM), penicillin/streptomycin (100 U/ml) and 25 AR TIC LE IN PR ES S μM Chloroquine (Sigma-Aldrich)), 80 %-confluent HEK-293T cells in 150 mm plates were cotransfected with 37.5 μg gRNA lentiviral plasmids, 20 μg viral packaging proteins plasmid (Pax2, Addgene #12260) and 9 μg VSV-G envelope proteins plasmid (Addgene #12259,) using a calcium phosphate transfection kit (Takara Bio). .. Following 16 hours of incubation at 37°C with 5% CO2, the medium was replaced with collection media (High-glucose DMEM (Gibco) supplemented with non-essential amino acids (NEAA, Gibco, 1x), 10% FBS (Gibco), GlutaMAX (2 mM), penicillin/streptomycin (100 U/ml) and 1 mM sodium butyrate (SigmaAldrich)) and incubated for another 30 hours.

    Plasmid Preparation:

    Article Title: MER57E3 transposable elements regulate gene expression in a human cell model of neural development.
    Article Snippet: .. Upon 2 hour incubation with Chloroquine-supplemented media (High-glucose DMEM (Gibco) supplemented with non-essential amino acids (NEAA, Gibco, 1x), 10% FBS (Gibco), GlutaMAX (2 mM), penicillin/streptomycin (100 U/ml) and 25 AR TIC LE IN PR ES S μM Chloroquine (Sigma-Aldrich)), 80 %-confluent HEK-293T cells in 150 mm plates were cotransfected with 37.5 μg gRNA lentiviral plasmids, 20 μg viral packaging proteins plasmid (Pax2, Addgene #12260) and 9 μg VSV-G envelope proteins plasmid (Addgene #12259,) using a calcium phosphate transfection kit (Takara Bio). .. Following 16 hours of incubation at 37°C with 5% CO2, the medium was replaced with collection media (High-glucose DMEM (Gibco) supplemented with non-essential amino acids (NEAA, Gibco, 1x), 10% FBS (Gibco), GlutaMAX (2 mM), penicillin/streptomycin (100 U/ml) and 1 mM sodium butyrate (SigmaAldrich)) and incubated for another 30 hours.

    Transfection:

    Article Title: MER57E3 transposable elements regulate gene expression in a human cell model of neural development.
    Article Snippet: .. Upon 2 hour incubation with Chloroquine-supplemented media (High-glucose DMEM (Gibco) supplemented with non-essential amino acids (NEAA, Gibco, 1x), 10% FBS (Gibco), GlutaMAX (2 mM), penicillin/streptomycin (100 U/ml) and 25 AR TIC LE IN PR ES S μM Chloroquine (Sigma-Aldrich)), 80 %-confluent HEK-293T cells in 150 mm plates were cotransfected with 37.5 μg gRNA lentiviral plasmids, 20 μg viral packaging proteins plasmid (Pax2, Addgene #12260) and 9 μg VSV-G envelope proteins plasmid (Addgene #12259,) using a calcium phosphate transfection kit (Takara Bio). .. Following 16 hours of incubation at 37°C with 5% CO2, the medium was replaced with collection media (High-glucose DMEM (Gibco) supplemented with non-essential amino acids (NEAA, Gibco, 1x), 10% FBS (Gibco), GlutaMAX (2 mM), penicillin/streptomycin (100 U/ml) and 1 mM sodium butyrate (SigmaAldrich)) and incubated for another 30 hours.



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